乳腺癌和纤维瘤中RECKmRNA和MMP9mRNA的表达差异及意义

The expression of the RECK mRNA and MMP9 mRNA in the breast cancer and fibroadenoma tissues

摘要:

摘要：目的
探讨RECKmRNA和MMP9mRNA在乳腺良、恶性肿瘤中的表达和意义。
方法 抽取乳腺肿瘤组织总RNA,采用半定量逆转录-聚合酶链反应(RTPCR) 检测39例乳腺癌和14例乳腺纤维腺瘤组织中RECKmRNA和MMP9mRNA的表达，分析其关系。
结果 乳腺癌组织RECK mRNA（与内参比值）为0.625±0.199，较纤维瘤组织的0.778±0.278明显降低(P<0.05) , MMP9 mRNA在癌中为1.342±0.168，比纤维瘤的0.790±0.157明显升高（P<0. 01）。
结论 RECK作为一种抑癌基因,在乳腺良恶性肿瘤中表达呈负相关，可抑制MMP9的表达,从而抑制乳腺癌的发生和转移。

Abstract:

Abstract:Objective
To investigate the expression level of RECK mRNA and MMP9mRNA in breast cancer(BC) and fibroadenoma tissues and its significance.
Methods The mRNA level of tissues of 39 cases of breast cancer and 14 cases of fibroadenoma was measured by RTPCR technique and its correlation was analyzed.
Results The mRNA level of RECK gene in breast cancer tissues was markedly lower than that in fibroadenoma tissues, while the MMP9 level was higher in BC tissues.
Conclusions RECK is a tumor suppressor gene, the expression in benign and malignant breast tissues is a negative relationship. BECK can inhibit the expression of MMP9 and thus, inhibit the growth and metastasis of breast cancer.