大鼠原位小肠移植模型的建立与改进

A modified model of orthotopic small intestinal transplantation in rats

摘要:

目的:探讨建立一种简便稳定存活率高的大鼠原位小肠移植模型。方法:整块切取带有腹主动脉和肠系膜上静脉并门静脉的节段小肠, 术中原位冷灌注, 4℃乳酸林格液保存。动脉吻合采用显微外科技术行供体腹主动脉对受体腹主动脉的端侧吻合,利用Cuff套管技术将供体的门静脉与受体的左肾静脉端端吻合。移植肠远、近端分别与受体肠行端端吻合。结果:建立小肠移植模型16次，动脉、静脉吻合时间分别为(25±5)min和（4±1）min。16只受体鼠中13只存活超过5d, 平均存活（10.35±2.84）d, 最长存活时间为21d。结论:移植肠的获取、血管吻合技术、肠吻合技术和维持良好的血容量是手术成功的关键。该模型的成功建立，为小肠移植的基础研究提供了良好的动物模型。

Abstract:

Abstract:Objective:To establish a simple and stable model of orthotopic small intestinal transplantation (OIT) in rats. Methods:The harvested en bloc segmental intestine consisted of donor abdominal aorta with superior mesenteric artery and portal vein. After in situ infusion with lactated Ringer′s solution , the graft was stored in 4℃ lactated Ringer′s solution. The segmental intestine transplantation was performed by end-to-side anastomosis of donor abdominal aorta to recipient abdominal aorta . The donor portal vein was anastomosed to the recipient left renal vein by "cuff anastomosis". The graft was anastomosed orthotopically by continuous sutures. Results:Sixteen intestine transplantations were, performed the average time for the arterial and venous anastomosis was 25±5 min and 4±1 min,respectively. Among the 16 recipient rats, 13 survived more than 5 days. The average survival time was 10.35±2.84 days; the longest survival time was 21 days. Conclusions:Graft harvesting, the technique of vascular and intestinal anastomosis and the maintenance of adequate blood volume are the key points for success of the operation. This successfully established model can serve as an excellent animal model for basic research of small intestinal trasplantation.