Ang2,HIF-1α及VEGF对肝癌血管形成的影响

Effect of angiopoietin2,hypoxia inducible factor-1α and vascular endothelial growth factor on angiogenesis in human hepatocellular carcinoma

摘要:

摘要：目的:探讨促血管生成素2（Ang2）、缺氧诱导因子1α（HIF-1α）及血管内皮生长因子（VEGF）与肝细胞癌血管形成的关系。方法:检测52例肝癌组织中Ang2，HIF-1α及VEGF mRNA及蛋白的表达，对共表达的肝癌组织进行微血管计数。结果:RT-PCR 显示,52例肝癌组织中有38例共表达Ang2mRNA，HIF-1αmRNA 和VEGF mRNA，且两两之间呈明显正相关(分别为r=0.783，P<0.01；r=0.427，P<0.05；r=0.433，P<0.05)；免疫组化发现,52例肝癌组织36例共表达Ang2，HIF-1α和VEGF蛋白。共表达Ang2 mRNA，HIF-αmRNA 和VEGF蛋白的38例肝癌组织中,平均微血管数［(45.4±8.90) 个/HP］,明显高于非共表达组［(13.6±3.30)个/HP］（P<0.05)。结论:Ang2，HIF-1α和VEGF与肝癌的新生血管形成有关；肿瘤组织缺氧可能是其始动因素。

Abstract:

Abstract:Objective:To investigate the relationship between hypoxia inducible factor-1α、angiopoietin2 and vascular endothelial growth factor and angiogenesis in hepatocellular carcinoma (HCC). Methods:The expression of hypoxia inducible factor-1α、angiopoietin2 and vascular endothelial growth factor mRNA was detected in 52 HCC surgical specimens. And microvessel density (MVD) in tissue specimens of patients with coexpression of the parameters was examined. Results:Of the 52 surgical specimens, 36 cases had over expression of HIF-α、angiopoietin and VEGF protein, and coexpression of HIF-α and angiopoietin and VEGF mRNA in 38 of 52 cases. The expression of HIF-α、angiopoietin was related with the expression of VEGF (r1= 0.783，P<0.01，r2=0.427，P<0.05，r3=0.433，P<0.05) . In the cases of over expression of HIF-α、angiopoietin and VEGF protein and mRNA, the average MVD was［（45.4 ±8.90）/HP］ significantly higher than that of cases with non coexpression group［(13.6±3.30)/HP］（P<0.05). Conclusions:HIF-α、angiopoietin and VEGF are possible angiogenic factors in the angiogenesis in HCC. Tumor tissue hypoxia may be the initiating factor.