乳腺癌患者外周血中T细胞亚群与淋巴结转移和组织学分级的关系

Relations of peripheral blood T cell subsets with lymph node metastasis and histological grade in breast cancer patients

摘要:

目的：探讨乳腺癌患者外周血中T细胞亚群的变化及其与淋巴结转移和组织学分级的关系。 方法：用流式细胞术检测86例乳腺癌患者以及20例乳腺腺病患者外周血T细胞亚群的百分率。 结果：乳腺癌患者外周血总T细胞与CD4+T细胞百分数与腺病患者无统计学差异（均P>0.05），但CD8+T细胞百分数低于腺病患者（P<0.05）。乳腺癌患者中，淋巴结转移者CD4+T细胞百分数高于无淋巴结转移者（P<0.05）；CD8+T细胞百分数随组织学分级增加而升高（P<0.05）。 结论：乳腺癌患者存在细胞免疫功能紊乱，外周血中CD4+T细胞、CD8+T细胞比例的变化分别与淋巴结转移、组织学分级密切相关。监测外周血T细胞亚群的变化，有助于病情及预后的判断。

Abstract:

Objective: To investigate the alterations of peripheral blood T cell subsets and their relations with lymph node metastasis and histological grade in breast cancer patients. Methods: The percentages of T cell subsets in peripheral blood in 86 patients with breast cancer and 20 patients with mammary adenosis were determined by flow cytometry. Results: There was no significant difference in percentages of peripheral blood total T cells and CD4+ T cells between breast cancer patients and mammary adenosis patients (both P>0.05), but the percentage of peripheral blood CD8+ T cells in breast cancer patients was lower than that of mammary adenosis patients (P<0.05). In breast cancer patients, the percentage of CD4+ T cells in cases with lymph node metastasis was higher than that of cases without lymph node metastasis (P<0.05); the percentage of CD8+ T cells was increased with histological grade increase (P<0.05). Conclusion: There are cell-mediated immune disorders in breast cancer patients, and the alteration in proportion of CD4+ T cells and CD8+ T cells in peripheral blood is closely related to lymph node metastasis and tumor histological grade, respectively. Monitoring of the alterations in peripheral blood T cell subsets may be helpful for estimation of the disease status and prognosis.