硫酸锌对大鼠移植胰腺再灌注损伤的保护作用

Protective effects of zinc sulfate on the reperfusion injury following pancreaticoduodenal transplantation in rats

摘要:

Objective:To study the protective effects of the heat shock protein 70 （HSP70）induced by zinc sulfate on reperfusion injury following pancreaticoduodenal transplantation in rats.Methods :The homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was used. The ZnSO4 treated rats received the intravenous injection of Zn2+5 mins before and after operation at a dose of 5 mg/kg (Zn1 group),10 mg/kg(Zn2 group) and 15 mg/kg(Zn3 group), and the control group with the same volume of saline. The tissue concentration of HSP70 was determined using WesternBlot. In addition，blood sugar (BG) and serum concentration of amylase and lipase were examined 24h after transplantation, and the activity of myeloperoxidase (MPO) in the pancrease graft was measured at the same time. Histological observation was performed.Results:Light microscopic studies showed that histomorphological changes of pancreas in Zn2 group and Zn1 group were much less than those in control group and Zn3 group. The value of BG and serum lipase and MPO in Zn2 group ＜ Zn1 group <control group < Zn3 group(P＜0.05). Conclusions:Zn2+(at the dose of 5 mg /kg and 10 mg /kg) can increase the amount of HSP. Zn2+has protective effects on the ischemia/reperfusion injury of pancreaticoduodenal transplantation, but the effect will disappear when the dose is over high.

Abstract:

胰腺/移植； 再灌注； 锌； 热休克蛋白