Abstract:T-cell lymphoma invasion and metastasis-inducing factor 1 (TIAM1) is a guanine nucleotide exchange factor (GEF) that plays a crucial role in tumorigenesis and progression. Increasing evidence has demonstrated that TIAM1 is highly expressed in pancreatic cancer and is closely associated with tumor proliferation, invasion, metastasis, and poor prognosis. This review summarizes the current advances in understanding the biological functions and molecular mechanisms of TIAM1 in pancreatic cancer. Particular emphasis is placed on the TIAM1-Rac1 signaling axis and its crosstalk with Wnt/β-catenin and RhoA pathways, as well as its roles in cytoskeletal remodeling, epithelial–mesenchymal transition (EMT), and metabolic reprogramming. In addition, the potential involvement of TIAM1 in shaping the tumor immune microenvironment is discussed. TIAM1 may serve as a promising prognostic biomarker and therapeutic target, and further elucidation of its regulatory network could provide novel insights for precision therapy in pancreatic cancer.