Abstract:Background and Aims Single-incision laparoscopic cholecystectomy (LC) has become increasingly popular due to its minimal invasiveness and cosmetic advantages, but challenges remain in biliary identification and limited operative space. Indocyanine green (ICG) fluorescence navigation enables real-time visualization of the biliary tract and may enhance surgical safety and efficiency. This study aimed to evaluate the clinical application value of ICG fluorescence navigation in single-incision LC.Methods A retrospective analysis was conducted on 59 patients with benign gallbladder diseases who underwent elective single-incision LC at Yixing Hospital affiliated to Jiangsu University from January 2023 to December 2024. Patients were divided into a fluorescence group (n=27) and a white-light group (n=32) according to whether ICG fluorescence navigation was applied. The two groups were compared in terms of Calot's triangle dissection time, operative time, intraoperative blood loss, surgeon satisfaction, hospital stay, and postoperative complications.Results No significant differences were observed in baseline clinical characteristics between the two groups (all P>0.05). In the fluorescence group, the cystic duct, common hepatic duct, and common bile duct were all successfully visualized. Compared with the white-light group, the fluorescence group had significantly shorter Calot's triangle dissection time [(25.56±3.49) min vs. (38.81±5.59) min], shorter operative time [(44.67±3.06) min vs. (61.31±4.96) min], and less intraoperative blood loss [(13.44±1.70) mL vs. (14.50±2.11) mL] (all P<0.05), with significantly higher surgeon satisfaction (P<0.05). No intraoperative bile duct injury, conversion to three-port surgery, or postoperative complications occurred in either group.Conclusion ICG fluorescence navigation can significantly improve biliary identification efficiency in single-incision LC, shorten operative time, reduce blood loss, and enhance surgeon satisfaction, demonstrating promising clinical application prospects.